ABSTRACT
Objective: To study the chemical constituents in the barks and leaves of Cinnamomum cassia.Methods: The crude extracts of the barks and leaves of Cinnamomum cassia were extracted by ethanol, the chemical constituents of the crude extracts were separated and purified by chromatographic methods and identified by spectroscopic analysis.Results: Five lignans were separated from the barks and two lignans were separated from the leaves of Cinnamomum cassia as follows: (7S,8R)-dihydrodehydrodiconiferyl alcohol 9'-O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside (1), 1,2,3-propanetriol,1--3-methoxyphenyl-(1R,2R) (2), (6R,7R,8R)-7a-lyoniresinol (3), (6S,7R,8R)-7a-lyoniresinol (4), (6R,7S,8S)-7a-lyoniresinol (5),(+)-lariciresinol(6), (-)-4-epi-lyoniresinol (7).Conclusion: All the lignans are isolated from Cinnamomum cassia for the first time.
ABSTRACT
Objective:To simultaneously separate 5 phenylpropanoids from Cinnamomum cassia by semi-preparative HPLC, and explore their immunosuppressive activity. Methods:After extracted by ethanol, the ethyl acetate part of Cinnamomum cassia was isola-ted by semi-preparative HPLC. The separation was conducted on an Ultimate XB-C18 (250 mm × 10 mm, 5μm) semi-preparative chro-matographic column and the mobile phase was acetonitrile-water with gradient elution. The detection wavelength was 330 nm, and the flow rate was 2. 5 ml·min-1 . The sample volume was 0. 3 ml. MS and NMR spectroscopic analysis were used to determine the config-urations. A CCK-8 method was used to detect the immunosuppressive activity of phenylpropanoids. Results: Totally 5 phenylpro-panoids were separated from Cinnamomum cassia by the semi-preparative HPLC, and identified as erythro-guaiacylglycerol, (7R,8S)-syringoylglycerol, (7S,8S)-syringoylglycerol, 3-methoxyphenyl-acrylaldehyde and O-methoxy cinnamaldehyde. The inhibitory rates of T cells and B cells of the compound 3 was more than 20% at the concentration of 800μmol·L-1 . Conclusion:The method is conven-ient with good separation effect, which can simultaneously separate 5 phenylpropanoids from Cinnamomum cassia, and among them, the compound 3 shows immunosuppressive effect to some extent.